Luminol-dependent chemiluminescence of whole blood
نویسندگان
چکیده
منابع مشابه
Mechanisms of non-opsonized zymosan-induced and luminol-enhanced chemiluminescence in whole blood and isolated phagocytes.
A luminol-dependent non-opsonized zymosan-induced chemiluminescence method for phagocytes in small quantities of whole blood (40 microliters; final dilution: 1:14) is described. It was characterized with reference to cellular and humoral components, and also applied to isolated neutrophils, eosinophils and monocytes. Normal values for whole blood chemiluminescence and for neutrophils, eosinophi...
متن کاملEffect of phenothiazines on activated macrophage-induced luminol-dependent chemiluminescence.
The inhibitory effect of some phenothiazine neuroleptics (chlorpromazine, levomepromazine, thioridazine, promethazine and trifluoperazine) on the ability of rat peritoneal macrophages to produce O2- during phagocytosis was investigated. The superoxide radical release was estimated by measuring the luminol-dependent chemiluminescence (CL). The effect of drugs was studied in the concentration ran...
متن کاملModulating luminol-dependent chemiluminescence of neutrophils by flavones.
The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone-8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy deriv...
متن کاملHuman bronchoalveolar lavage cells and luminol-dependent chemiluminescence.
Human bronchoalveolar lavage cells from several different disease states were examined by the technique of zymosan-stimulated, luminol-dependent chemiluminescence. Light production correlated well with polymorphonuclear leucocyte contamination of the alveolar macrophage suspension but not with lymphocyte contamination. Regression analysis indicated that human alveolar macrophages produce little...
متن کاملChemiluminescence Determination of Atropine using Luminol-Hemin-H2O2 System
A sensitive and fast chemiluminescence (CL) method has been described for the direct determination of atropine. The method is based on the quenching effect of atropine on the CL signals of the luminol– H2O2 reaction catalyzed by hemin in an alkaline medium. Hemin could strongly enhance the chemiluminescence of the luminol–H2O2 system. The luminophor of the luminol–H2O2–hemin CL system was ident...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Ensho
سال: 1984
ISSN: 0389-4290,1884-4006
DOI: 10.2492/jsir1981.4.4_365